MLH3 Antibody (CL14096)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24597
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL14096
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for MLH3 Antibody (CL14096)
Immunogen
This antibody was designed to target the recombinant protein MLH3, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for MLH3 Antibody (CL14096)
Immunohistochemistry-Paraffin: MLH3 Antibody (CL14096) [NBP3-24597]
Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MLH3 Antibody (CL14096) [NBP3-24597]
Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MLH3 Antibody (CL14096) [NBP3-24597]
Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.
Applications for MLH3 Antibody (CL14096)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:20-1:50
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MLH3
Alternate Names
DNA mismatch repair protein Mlh3, HNPCC7, MGC138372, mutL (E. coli) homolog 3, mutL homolog 3 (E. coli), MutL protein homolog 3
Gene Symbol
MLH3
Additional MLH3 Products
Product Documents for MLH3 Antibody (CL14096)
Product Specific Notices for MLH3 Antibody (CL14096)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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