MLL3 Antibody (PSH02-11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32507
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH02-11
Concentration
1 mg/ml
Product Summary for MLL3 Antibody (PSH02-11)
Immunogen
Recombinant protein within human MLL3 aa 3,251-3,550 / 4,911. (Uniprot: Q8NEZ4)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
541 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MLL3 Antibody (PSH02-11)
Western Blot: MLL3 Antibody (PSH02-11) [NBP3-32507]
Western Blot: MLL3 Antibody (PSH02-11) [NBP3-32507] - Western blot analysis of MLL3 on different lysates with Rabbit anti-MLL3 antibody (NBP3-32507) at 1/1,000 dilution.Lane 1: U-2 OS cell lysate (20 ug/Lane)
Lane 2: MCF7 cell lysate (20 ug/Lane)
Lane 3: HCT 116 cell lysate (20 ug/Lane)
Lane 4: Rat skeletal muscle tissue lysate (40 ug/Lane)
Predicted band size: 541 kDa
Observed band size: 541 kDa
Exposure time: 1 minute 36 seconds;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32507) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for MLL3 Antibody (PSH02-11)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MLL3
Alternate Names
ALR-like protein, DKFZp686C08112, EC 2.1.1.43, FLJ12625, HALRMGC119851, histone-lysine N-methyltransferase MLL3, histone-lysine N-methyltransferase, H3 lysine-4 specific, Homologous to ALR protein, KIAA1506FLJ38309, KMT2C, KMT2CMGC119852, Lysine N-methyltransferase 2C, MGC119853, myeloid/lymphoid or mixed-lineage leukemia 3, Myeloid/lymphoid or mixed-lineage leukemia protein 3
Gene Symbol
KMT2C
Additional MLL3 Products
Product Documents for MLL3 Antibody (PSH02-11)
Product Specific Notices for MLL3 Antibody (PSH02-11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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