MLL5 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03559

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MLL5 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human MLL5 (NP_061152.3). VELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQHISKCKKAFREGSRKSSRVKGSAPEIDPSSDGSNFGWETKIKAWMDRYEEANNNQYSEGVQREAQRIALRLGNGNDKKEMNKSDLNTNNLLFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESMENINSGYETRRKKGKKDKDISK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MLL5 Antibody - Azide and BSA Free

Western Blot: MLL5 AntibodyAzide and BSA Free [NBP3-03559]

Western Blot: MLL5 AntibodyAzide and BSA Free [NBP3-03559]

Western Blot: MLL5 Antibody [NBP3-03559] - Western blot analysis of extracts of C2C12 cells, using (NBP3-03559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry: MLL5 Antibody - Azide and BSA Free [NBP3-03559]

Immunohistochemistry: MLL5 Antibody - Azide and BSA Free [NBP3-03559]

Immunohistochemistry: MLL5 Antibody [NBP3-03559] - Analysis of mouse brain using MLL5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MLL5 Antibody - Azide and BSA Free [NBP3-03559]

Immunohistochemistry-Paraffin: MLL5 Antibody - Azide and BSA Free [NBP3-03559]

Immunohistochemistry-Paraffin: MLL5 Antibody [NBP3-03559] - Paraffin-embedded human breast cancer using MLL5 antibody at dilution of 1:200 (40x lens).
View 2 more images

Applications for MLL5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MLL5

MLL5 is a divergent member of the Drosophila Trithorax related SET and PHD domain containing chromatin regulators that are involved in the regulation of transcriptional "memory" during differentiation. Human MLL5 is located on chromosome 7q22, that is frequently deleted in myeloid leukemias. Inactivation of the Mll5 gene in mice results in a reduction in the average representation of hematopoietic stem cells, and in functional impairment of long term hematopoietic repopulation potential under competitive conditions. Overexpression of the protein also inhibits cell cycle progression.

Alternate Names

EC 2.1.1.43, FLJ10078, HDCMC04P, histone-lysine N-methyltransferase MLL5, KMT2EFLJ14026, Lysine N-methyltransferase 2E, MGC70452, myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila), Myeloid/lymphoid or mixed-lineage leukemia protein 5

Gene Symbol

KMT2E

Additional MLL5 Products

Product Documents for MLL5 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MLL5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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