MLLT1 Antibody (3H2)
Novus Biologicals, part of Bio-Techne | Catalog # H00004298-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3H2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MLLT1 (NP_005925, 472 a.a. ~ 558 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GRRSPESCSKPEKILKKGTYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFSLDETTVRKLQSCLEAVA
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25481096). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
MLLT1 - myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for MLLT1 Antibody (3H2)
![Immunocytochemistry/ Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01]](https://resources.bio-techne.com/images/products/MLLT1-Antibody-3H2-Immunocytochemistry-Immunofluorescence-H00004298-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01]")
Immunocytochemistry/ Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01]
Immunocytochemistry/Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01] - Analysis of monoclonal antibody to MLLT1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: MLLT1 Antibody (3H2) [H00004298-M01] - Detection limit for recombinant GST tagged MLLT1 is approximately 0.1ng/ml as a capture antibody.
Western Blot: Mouse Monoclonal MLLT1 Antibody (3H2) [H00004298-M01]
Western Blot: Mouse Monoclonal MLLT1 Antibody (3H2) [H00004298-M01] - Western Blot showing amount of protein, cell lines tested (293T, over expression line 293T+hENL, and mESCs) and loading control blot using alpha-tubulin. Image from a verified customer review.Applications for MLLT1 Antibody (3H2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IF. IHC-P usage reported in scientific literature (PMID: 25481096).
Reviewed Applications
Read 1 review rated 3 using H00004298-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MLLT1
Alternate Names
ENLENL/MLL fusion, LTG19MLLT1/MLL fusion, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 1, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 1, protein ENL, YEATS domain-containing protein 1, YEATS1myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 1
Entrez Gene IDs
4298 (Human)
Gene Symbol
MLLT1
OMIM
159556 (Human)
UniProt
Additional MLLT1 Products
Product Documents for MLLT1 Antibody (3H2)
Product Specific Notices for MLLT1 Antibody (3H2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...