MMP-12 Antibody (SR03-23)

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344]

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 on different lysates with Rabbit anti-MMP-12 antibody at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344]

Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.