MMP-13 Antibody (8L8Q1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15346

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8L8Q1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-13 Antibodies »

Product Summary for MMP-13 Antibody (8L8Q1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MMP-13 (P45452). RGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-13 Antibody (8L8Q1)

Western Blot: MMP-13 Antibody (8L8Q1) [NBP3-15346]

Western Blot: MMP-13 Antibody (8L8Q1) [NBP3-15346]

Western Blot: MMP-13 Antibody (8L8Q1) [NBP3-15346] - Western blot analysis of extracts of various cell lines, using MMP-13 Rabbit mAb (NBP3-15346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (8L8Q1) [NBP3-15346]

Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (8L8Q1) [NBP3-15346]

Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (8L8Q1) [NBP3-15346] - Immunofluorescence analysis of HeLa cells using MMP-13 Rabbit mAb (NBP3-15346) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MMP-13 Antibody (8L8Q1) [NBP3-15346]

Immunohistochemistry-Paraffin: MMP-13 Antibody (8L8Q1) [NBP3-15346]

Immunohistochemistry-Paraffin: MMP-13 Antibody (8L8Q1) [NBP3-15346] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP-13 Rabbit mAb (NBP3-15346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for MMP-13 Antibody (8L8Q1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMP-13

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Gene Symbol

MMP13

Additional MMP-13 Products

Product Documents for MMP-13 Antibody (8L8Q1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-13 Antibody (8L8Q1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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