MMP-13 Antibody (VIIIA2)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-5919

Novus Biologicals, part of Bio-Techne
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NB110-5919
NB110-5919-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # VIIIA2

Concentration

0.4 mg/ml

View all MMP-13 Antibodies »

Product Specifications

Immunogen

Human recombinant collagenase-3 protein

Reactivity Notes

Rat reactivity reported in scientific literature (see Acta Odontol Scand. 2008). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Secreted

Specificity

Recognizes pro and acive forms of MMP 13.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MMP-13 Antibody (VIIIA2)

Immunohistochemistry-Paraffin: MMP-13 Antibody (VIIIA2) [NB110-5919]

Immunohistochemistry-Paraffin: MMP-13 Antibody (VIIIA2) [NB110-5919]

Immunohistochemistry-Paraffin: MMP-13 Antibody (VIIIA2) [NB110-5919] - Human placenta stained with MMP-13 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.

Applications for MMP-13 Antibody (VIIIA2)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:12.5 - 1:25

Immunoprecipitation

1:25 - 1:50

Western Blot

2 - 4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4) and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

0.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MMP-13

Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Entrez Gene IDs

4322 (Human); 17386 (Mouse); 100008685 (Rabbit)

Gene Symbol

MMP13

UniProt

P45452

Additional MMP-13 Products

Product Documents for MMP-13 Antibody (VIIIA2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-13 Antibody (VIIIA2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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