MMP-14/MT1-MMP Antibody (S05-6H5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19491

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19491-100ul
NBP3-19491-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-6H5

Concentration

0.3 mg/ml

View all MMP-14/MT1-MMP Antibodies »

Product Summary for MMP-14/MT1-MMP Antibody (S05-6H5)

Immunogen

A synthetic peptide of human MMP-14/MT1-MMP (Uniprot # P50281)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-14/MT1-MMP Antibody (S05-6H5)

Western Blot: MMP-14/MT1-MMP Antibody (S05-6H5) [NBP3-19491]

Western Blot: MMP-14/MT1-MMP Antibody (S05-6H5) [NBP3-19491]

Western Blot: MMP-14/MT1-MMP Antibody (S05-6H5) [NBP3-19491] - Western blot detection of MMP-14/MT1-MMP in CHO-K1 cell lysates using NBP3-19491 (1:1000 diluted). Predicted band size: 66kDa. Observed band size: 66kDa.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (S05-6H5) [NBP3-19491]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (S05-6H5) [NBP3-19491]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (S05-6H5) [NBP3-19491] - Immunohistochemistry of MMP-14/MT1-MMP in paraffin-embedded Human breast cancer tissue using MMP-14/MT1-MMP Rabbit mAb at dilution 1/100

Applications for MMP-14/MT1-MMP Antibody (S05-6H5)

Application
Recommended Usage

Immunohistochemistry

1/50

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (S05-6H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-14/MT1-MMP Antibody (S05-6H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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