MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB200-114
![Western Blot: MMP-2 Antibody (8B4)(Pro and Active)BSA Free [NB200-114]](https://resources.bio-techne.com/images/products/MMP-2-Antibody-8B4-Pro-and-Active-Western-Blot-NB200-114-img0005.jpg "Western Blot: MMP-2 Antibody (8B4)(Pro and Active)BSA Free [NB200-114]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8B4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Activated recombinant human MMP-2.
Specificity
This is specific for pro and active MMP2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free
Western Blot: MMP-2 Antibody (8B4)(Pro and Active)BSA Free [NB200-114]
Western Blot: MMP-2 Antibody (8B4) - (Pro and Active) [NB200-114] - Analysis of MMP2 expression in ProMMP2 (1), Active MMP2 (2) and human pressure ulcer biopsy (3).![Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free [NB200-114]](https://resources.bio-techne.com/images/products/MMP-2-Antibody-8B4-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-114-img0006.jpg "Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free [NB200-114]")
Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free [NB200-114]
Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) [NB200-114] - Detection of MMP-2 staining in FFPE human ovary tissue. Primary antibody was used at 2.5 ug/ml with the avidin-biotin-peroxidase detection method.Applications for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free
Application
Recommended Usage
ELISA
0.03 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
2 ug/ml
Immunohistochemistry-Frozen
2ug/ml
Immunohistochemistry-Paraffin
2 ug/ml
Knockdown Validated
reported in scientific literature (PMID 31439546)
Western Blot
1-2ug/ml
Application Notes
In Western blot, a band is seen at 70 and 62kDa representing the MMP2 band-pair. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MMP-2
Long Name
Matrix Metalloproteinase 2
Alternate Names
Gelatinase A, MMP2
Gene Symbol
MMP2
Additional MMP-2 Products
Product Documents for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free
Product Specific Notices for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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