MMP-3 Antibody (MMP3/2655)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79730

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MMP3/2655

Concentration

0.2 mg/ml

View all MMP-3 Antibodies »

Product Summary for MMP-3 Antibody (MMP3/2655)

Immunogen

Recombinant fragment of human MMP-3 protein (around aa 236-363) (exact sequence is proprietary) (Uniprot: P08254)

Localization

Cytoplasmic, Extracellular Matrix

Marker

Marker of Metastasis and Rheumatoid Arthritis

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79865)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MMP-3 Antibody (MMP3/2655)

Western Blot: MMP-3 Antibody (MMP3/2655) [NBP2-79730]

Western Blot: MMP-3 Antibody (MMP3/2655) [NBP2-79730]

Western Blot: MMP-3 Antibody (MMP3/2655) [NBP2-79730] - Western Blot Analysis of Human Raji, HEK293, and HT-29 cell lysate using MMP-3 Antibody (MMP3/2655).
Protein Array: MMP-3 Antibody (MMP3/2655) [NBP2-79730]

Protein Array: MMP-3 Antibody (MMP3/2655) [NBP2-79730]

Protein Array: MMP-3 Antibody (MMP3/2655) [NBP2-79730] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MMP-3 Antibody (MMP3/2655). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for MMP-3 Antibody (MMP3/2655)

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MMP-3

Effective inhibitors of matrix metalloproteinases (MMPs), a family of connective tissue-degrading enzymes, could be useful for the treatment of diseases such as cancer, multiple sclerosis, and arthritis (1). Matrix metalloproteinase-3 (MMP-3) is a protein involved in tumor invasion and metastasis. MMP3 (stromelysin) is a secreted metalloprotease with a wide range of substrate specificities. MMP3 is synthesized in a preproenzyme form with a calculated size of 54 kDa and a 17-amino acid long signal peptide. Data indicate that the expression and the possible involvement of secreted metalloproteases in tumorigenesis result from a specific interaction between the transforming factor and the target cell, which may vary in different species (2). In neural studies, matrix metalloproteinase-3 (MMP-3) was newly induced and activated in stressed dopamine cells, and the active form of MMP-3 (actMMP-3) was released into the medium. The released actMMP-3, as well as catalytically active recombinant MMP-3 (cMMP-3) led to microglial activation and superoxide generation in microglia and enhanced DA cell death. (3).

Long Name

Matrix Metalloproteinase 3

Alternate Names

MMP3, Stromelysin 1

Gene Symbol

MMP3

UniProt

P08254

Additional MMP-3 Products

Product Documents for MMP-3 Antibody (MMP3/2655)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-3 Antibody (MMP3/2655)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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