MMP-9 Antibody (CL0538)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52948

Novus Biologicals, part of Bio-Techne
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NBP2-52948
NBP2-52948-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0538

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MMP-9 Antibodies »

Product Summary for MMP-9 Antibody (CL0538)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADI

Epitope

VPDLGRFQTF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MMP-9 Antibody (CL0538)

Western Blot: MMP-9 Antibody (CL0538) [NBP2-52948]

Western Blot: MMP-9 Antibody (CL0538) [NBP2-52948]

Western Blot: MMP-9 Antibody (CL0538) [NBP2-52948] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MMP9 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948]

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948]

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human uterus shows absence of immunoreactivity.
Immunohistochemistry: MMP-9 Antibody (CL0538) [NBP2-52948]

Immunohistochemistry: MMP-9 Antibody (CL0538) [NBP2-52948]

Immunohistochemistry: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of lymphoid cells.
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Applications for MMP-9 Antibody (CL0538)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-9

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for MMP-9 Antibody (CL0538)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-9 Antibody (CL0538)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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