MMP-9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85575

Novus Biologicals, part of Bio-Techne
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NBP1-85575
NBP1-85575-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MMP-9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%). Human reactivity reported in scientific literature (PMID: 23897811).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-9 Antibody

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-85575] - Staining in human bone marrow and cerebral cortex tissues using anti-MMP9 antibody. Corresponding MMP9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody [NBP1-85575]

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody [NBP1-85575]

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody [NBP1-85575] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-85575] - Staining of human bone marrow shows high expression.
View 1 more images

Applications for MMP-9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 23897811). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-9

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for MMP-9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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