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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid peptide near the center of human MMP9. The immunogen is located within amino acids 260 - 310 of MMP9 . Amino Acid Squence: PSERLYTQDGNADGK

Specificity

At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-9 Antibody - BSA Free

Western Blot: MMP-9 AntibodyBSA Free [NBP2-41233]

Western Blot: MMP-9 AntibodyBSA Free [NBP2-41233]

Western Blot: MMP-9 Antibody [NBP2-41233] - Analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 ug/ml.
MMP-9 Antibody - BSA Free

Immunohistochemistry: MMP-9 Antibody - BSA Free [NBP2-41233] -

Immunohistochemistry: MMP-9 Antibody - BSA Free [NBP2-41233] - Immunohistochemistry of MMP-9 in human lung tissue with MMP-9 antibody at 2.5 u/mL.
MMP-9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP2-41233] -

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP2-41233] - Immunofluorescence of MMP-9 in human lung tissue with MMP-9 antibody at 20 ug/mL.

Applications for MMP-9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-9

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for MMP-9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MMP-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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