MNAT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06477
![Western Blot: MNAT1 Antibody [NBP3-06477]](https://resources.bio-techne.com/images/products/MNAT1-Antibody-Western-Blot-NBP3-06477-img0001.jpg "Western Blot: MNAT1 Antibody [NBP3-06477]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human MNAT1 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for MNAT1 Antibody
Western Blot: MNAT1 Antibody [NBP3-06477]
Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa![Immunocytochemistry/ Immunofluorescence: MNAT1 Antibody [NBP3-06477]](https://resources.bio-techne.com/images/products/MNAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06477-img0002.jpg "Immunocytochemistry/ Immunofluorescence: MNAT1 Antibody [NBP3-06477]")
Immunocytochemistry/ Immunofluorescence: MNAT1 Antibody [NBP3-06477]
Immunocytochemistry/Immunofluorescence: MNAT1 Antibody [NBP3-06477] - Staining of MNAT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MNAT1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.![Immunoprecipitation: MNAT1 Antibody [NBP3-06477]](https://resources.bio-techne.com/images/products/MNAT1-Antibody-Immunoprecipitation-NBP3-06477-img0003.jpg "Immunoprecipitation: MNAT1 Antibody [NBP3-06477]")
Immunoprecipitation: MNAT1 Antibody [NBP3-06477]
Immunoprecipitation: MNAT1 Antibody [NBP3-06477] - Lane A:0.5 mg HELA Whole Cell Lysate4 uL anti-MNAT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MNAT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :35 kDaApplications for MNAT1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MNAT1
Alternate Names
CAP35, CDK7/cyclin-H assembly factor, cyclin G1 interacting protein, Cyclin-G1-interacting protein, MAT1TFB3, Menage a trois, menage a trois 1 (CAK assembly factor), menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis), p35, p36, RING finger protein 66, RING finger protein MAT1, RNF66CDK-activating kinase assembly factor MAT1
Gene Symbol
MNAT1
Additional MNAT1 Products
Product Specific Notices for MNAT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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