MNK1 Antibody (2F12)

Name: MNK1 Antibody (2F12)
Brand: Novus Biologicals
SKU: H00008569-M14

Novus Biologicals, part of Bio-Techne | Catalog # H00008569-M14

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MAP kinase interacting serine/threonine kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MNK1 Antibody (2F12)

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Old wild type and p38 alpha knock out livers were Western blotted for MNK1 and phosphorylated MNK1 on Thr197/202. Alpha tubulin was used as a loading control. Densitometric quantification of p-MNK-1(T197/202)/MNK1 were determined. Data are shown as mean +/- SD. **P < 0.01 WT versus KO. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171738) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14]

Immunocytochemistry/Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14]

Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - detection against Immunogen (77.26 KDa) .

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of MKNK1 expression in Raw 264.7. (Isoform: 51.342, 47.402, 39.103 kD)

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] -

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - p38 alpha-mediated phosphorylation pathways.a. Old wild type & p38 alpha knock out livers were Western blotted for phosphorylated MKK3/6 & MKK4, for phosphorylated p38 & total levels of p38 alpha. Densitometric quantification of p-MKK3/6 (S189/207)/ alpha-tubulin, p-MKK4 (S257/T261)/a tubulin were done. b. Old wild type & p38 alpha knock out livers were Western blotted for MNK1 & phosphorylated MNK1 on Thr197/202, for MK2 & phosphorylated MK2 on Thr334 & Thr222, for AKT & phosphorylated AKT on Ser473, for GSK3 beta & phosphorylated GSK3 beta on serine 9 & for HSP27 & phosphorylated HSP27 on Ser 82. alpha-tubulin was used as a loading control. Densitometric quantification of p-MNK-1(T197/202)/MNK1, p-MK2 (T334)/MK2, p-MK2 (T222) /MK2, p-AKT(S473)/AKT, p-GSK3 beta(S9)/GSK3 beta & p-HSP27(S82)/HSP27 were determined. Data are shown as mean ± SD. **P < 0.01 WT versus KO. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0171738), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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