Mono- and polyubiquitinylated conjugates Antibody (UBCJ2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06879

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # UBCJ2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Mono- and polyubiquitinylated conjugates Antibodies »

Product Summary for Mono- and polyubiquitinylated conjugates Antibody (UBCJ2)

Immunogen

Poly-ubiquitinylated lysozyme.

Specificity

Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) has been shown to detect K48 and K63-linked mono- and polyubiquitinylated proteins. Although not tested, in theory, this antibody should react to all mono- and polyubiquitinylated protein conjugates, regardless the linkage (K6, K11, K27, K29, K33, K48 or K63).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Mono- and polyubiquitinylated conjugates Antibody (UBCJ2)

Western Blot: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879]

Western Blot: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879]

Western Blot: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879] - Western Blot analysis of Mono- and polyubiquitinylated conjugates Antibody (HRP conjugated) detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.
Immunocytochemistry/ Immunofluorescence: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879]

Immunocytochemistry/ Immunofluorescence: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879]

Immunocytochemistry/Immunofluorescence: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879] - Immunocytochemistry analysis of ubiquitinylated protein conjugates using FITC labeled Mono- and polyubiquitinylated conjugates Antibody. HeLa cells, pre-treated with proteasome inhibitor MG-132 at 5 uM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control; Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.
Flow Cytometry: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879]

Flow Cytometry: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879]

Flow Cytometry: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879] - Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates Antibody (FITC conjugated), at a concentration of 10ug/ml, side-by-side comparison to another Mono- and polyubiquitinylated conjugates clone (FK2) (fluorescein labeled).

Applications for Mono- and polyubiquitinylated conjugates Antibody (UBCJ2)

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:1000
Application Notes
Suggested dilutions/conditions may not be appropriate for all applications. Optimal conditions must be determined individually for each application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mono- and polyubiquitinylated conjugates

Alternate Names

HEL-S-50, polyubiquitin B, polyubiquitin-B, UBB, UBC, ubiquitin B, ubiquitin C

Gene Symbol

UBC

Additional Mono- and polyubiquitinylated conjugates Products

Product Documents for Mono- and polyubiquitinylated conjugates Antibody (UBCJ2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mono- and polyubiquitinylated conjugates Antibody (UBCJ2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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