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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide, which maps to the region between residues 600-700 of human zinc finger, CW-type with coiled-coil domain 1 using the numbering given in entry NP_055756.1 (GeneID 22880).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MORC2 Antibody

Western Blot: MORC2 Antibody [NB600-236]

Western Blot: MORC2 Antibody [NB600-236]

Western Blot: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MORC2 antibody [NB600-236] used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: MORC2 Antibody [NB600-236]

Immunoprecipitation: MORC2 Antibody [NB600-236]

Immunoprecipitation: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MORC2 antibody NB600-236 used for IP at 3 ug per reaction. MORC2 was also immunoprecipitated by rabbit anti-MORC2 antibody NB600-237. For blotting immunoprecipitated MORC2, NB600-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MORC2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg of lysate

Western Blot

1:2500-1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~111 kDa, representing ZCWCC1 protein. May be used for immunoprecipitation, as well. Suggested working dilutions: * Western Blot - 1:2,500-1:10,000 Immunoprecipitation - 2-5 ug/mg lysate Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MORC2

This is a zinc-finger CW-type coil-coiled domain 1 protien. In humans it is mapped to chromosome 22q12.2. It is highly expressed in smooth muscle, pancreas and testis.

Alternate Names

AC004542.C22.1, KIAA0852, MORC family CW-type zinc finger 2, MORC family CW-type zinc finger protein 2, ZCW3, ZCWCC1, Zinc finger CW-type coiled-coil domain protein 1, zinc finger, CW type with coiled-coil domain 1, zinc finger, CW-type with coiled-coil domain 1

Entrez Gene IDs

22880 (Human)

Gene Symbol

MORC2

Additional MORC2 Products

Product Documents for MORC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MORC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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