Mouse ADAM10 Ectodomain Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB946R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 139712
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Thr215-Glu673
Accession # O35598
Thr215-Glu673
Accession # O35598
Specificity
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse ADAM10 Ectodomain Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ADAM10
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
References
- Rooke, J. et al. (1996) Science 273:1227.
- Pan, D. and Rubin, G.M. (1997) Cell 90:271.
- Qi, H. et al. (1999) Science 283:91.
- Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
- Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
- Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
- Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
- Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
- Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Alternate Names
CD156c, Kuzbanian, MADM
Gene Symbol
ADAM10
UniProt
Additional ADAM10 Products
Product Documents for Mouse ADAM10 Ectodomain Alexa Fluor® 647-conjugated Antibody
Product Specific Notices for Mouse ADAM10 Ectodomain Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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