Mouse alpha-Fetoprotein/AFP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5369
Key Product Details
Species Reactivity
Mouse
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 870826
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse alpha-Fetoprotein/AFP
Lys19-Val605
Accession # P02772
Lys19-Val605
Accession # P02772
Specificity
Detects mouse alpha-Fetoprotein in direct ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse alpha-Fetoprotein/AFP Antibody
Mouse alpha-Fetoprotein/AFP ELISA Standard Curve.
Recombinant Mouse alpha-Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha-Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Mouse alpha-Fetoprotein/AFP Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse alpha‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse alpha-Fetoprotein/AFP DuoSet ELISA Kit (Catalog # DY5369-05) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP
Alternate Names
AFP, DSCAM2
Gene Symbol
AFP
UniProt
Additional alpha-Fetoprotein/AFP Products
Product Documents for Mouse alpha-Fetoprotein/AFP Antibody
Product Specific Notices for Mouse alpha-Fetoprotein/AFP Antibody
For research use only
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