Mouse Angiopoietin-like Protein 2/
ANGPTL2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1444
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Angiopoietin-like 2
Pro23-His493
Accession # Q9R045
Pro23-His493
Accession # Q9R045
Specificity
Detects mouse Angiopoietin-like Protein 2/ANGPTL2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) ANGPTL6 and rmANGPTL1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Angiopoietin-like Protein 2/ANGPTL2 Antibody
Detection of Mouse Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot.
Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin-like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin-like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Angiopoietin-like Protein 2/ANGPTL2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15), skeletal muscle, and thymus
Sample: Immersion fixed frozen sections of mouse embryo (E15), skeletal muscle, and thymus
Western Blot
2 µg/mL
Sample: Mouse uterus tissue and MEF mouse embryonic feeder cells
Sample: Mouse uterus tissue and MEF mouse embryonic feeder cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 2/ANGPTL2
Alternate Names
ANGPTL2, ANGRP2
Gene Symbol
ANGPTL2
UniProt
Additional Angiopoietin-like Protein 2/ANGPTL2 Products
- All Products for Angiopoietin-like Protein 2/ANGPTL2
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Product Documents for Mouse Angiopoietin-like Protein 2/ANGPTL2 Antibody
Product Specific Notices for Mouse Angiopoietin-like Protein 2/ANGPTL2 Antibody
For research use only
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