Mouse Autoimmune Regulator/AIRE Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6184

R&D Systems, part of Bio-Techne
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AF6184
AF6184-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Autoimmune Regulator/AIRE Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Autoimmune Regulator/AIRE
Ser476-Ser552
Accession # Q9Z0E3

Specificity

Detects mouse Autoimmune Regulator/AIRE in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Autoimmune Regulator/AIRE Antibody

Detection of Mouse Autoimmune Regulator/AIRE antibody by Western Blot.

Detection of Mouse Autoimmune Regulator/AIRE by Western Blot.

Western blot shows lysates of mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse Autoimmune Regulator/AIRE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Autoimmune Regulator/AIRE at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Mouse Autoimmune Regulator/AIRE Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse thymus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Autoimmune Regulator/AIRE

AIRE (Autoimmune regulator; also APECED) is a 58-60 kDa transcriptional regulator that exhibits tight regulation of its expression. It is widely expressed in lymphoid organs, but is most pronounced in thymic medullary epithelium, where it induces the expression of organ specific antigens that are used to sequester and delete autoreactive lymphocytes. Mouse AIRE is 552 amino acids (aa) in length. It contains multiple domains, including an HSR domain with two LXXLL motifs (aa 1-106), an NLS (aa 114-134), one SAND domain (aa 182-282) and two PHD zinc-finger regions (aa 298-345; 434-475). AIRE will homodimerize and tetramerize. There are multiple potential isoforms that involve various combinations of deletions of aa 265-268, 368-426 and Lys296, plus a 42 aa substitution for aa 368-552. Over aa 476-552, mouse AIRE shares 91% and 65% aa identity with rat and human AIRE, respectively.

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Entrez Gene IDs

326 (Human); 11634 (Mouse); 294328 (Rat)

Gene Symbol

AIRE

UniProt

Q9Z0E3

Additional Autoimmune Regulator/AIRE Products

Product Documents for Mouse Autoimmune Regulator/AIRE Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Autoimmune Regulator/AIRE Antibody

For research use only

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