Mouse B7-1/CD80 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7401
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Val38-Asn246
Accession # Q00609
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse B7-1/CD80 Antibody
Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes, (A) treated with 200 ng/mL LPS for 16 hr, or (B) resting, were stained with Rabbit Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB7401) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Rat anti-Mouse B220 PE-conjugated Monoclonal Antibody (FAB1217P). Quadrant markers were set based on isotype control antibody (MAB1050, data not shown). Staining was done using our Staining Membrane-associated Proteins protocol.Applications for Mouse B7-1/CD80 Antibody
Flow Cytometry
Sample: Mouse splenocytes treated with 200 ng/mL LPS
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-1/CD80
B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
References
- Azuma, M. et al. (1993) Nature 366:76.
- Freeman, G.J. et al. (1993) Science 262:909.
- Freeman, G. et al. (1991) J. Exp. Med. 174:625.
- Selvakumar, A. et al. (1993) Immunogenetics 38:292.
- Chen, C. et al. (1994) J. Immunol. 152:4929.
- Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Alternate Names
Gene Symbol
UniProt
Additional B7-1/CD80 Products
Product Documents for Mouse B7-1/CD80 Antibody
Product Specific Notices for Mouse B7-1/CD80 Antibody
For research use only