Mouse BCMA/TNFRSF17 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB593
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 161616
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BCMA
Met1-Thr49
Accession # O88472
Met1-Thr49
Accession # O88472
Specificity
Detects mouse BCMA in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) BCMA or rhTACI.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse BCMA/TNFRSF17 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2 - 8 μg/mL of this antibody will block 50% of the binding of 750 ng/mL of recombinant human APRIL to immobilized recombinant mouse BCMA/Fc Chimera coated at 1 μg/mL (100 μL/well). At 100 μg/mL, this antibody will block >90% of the binding. The specific conditions are described in the figure legend. In this application, the use of anti-mouse BCMA monoclonal antibody (Catalog # MAB5931), is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCMA/TNFRSF17
BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.
References
- Madry, C. et al. (1998) Int. Immunol. 10:1693.
- Gras, M. et al. (1995) Int. Immunol. 7:1093.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
- Marsters, S. et al. (2000) Curr. Biol. 10:785.
- Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Alternate Names
CD269, TNFRSF13A, TNFRSF17
Gene Symbol
TNFRSF17
UniProt
Additional BCMA/TNFRSF17 Products
Product Documents for Mouse BCMA/TNFRSF17 Antibody
Product Specific Notices for Mouse BCMA/TNFRSF17 Antibody
For research use only
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