Mouse BMP-6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6325
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-6
Ser372-His510
Accession # P20722
Ser372-His510
Accession # P20722
Specificity
Detects recombinant human and mouse BMP-6 in direct ELISAs and detects mouse BMP-6 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) BMP-5 and rmBMP-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse BMP-6 Antibody
Detection of Mouse BMP‑6 by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with mouse BMP-6. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Mouse BMP-6 (Catalog # 6325-BM) (10ng/lane) was included. A specific band was detected for BMP-6 at approximately 20kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.BMP‑6 in Mouse Embryo.
BMP-6 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to skeletal muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse BMP-6 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Western Blot
1 µg/mL
Sample: NS0 mouse myeloma cell line transfected with mouse BMP‑6 and Recombinant Mouse BMP‑6 (Catalog # 6325-BM)
Sample: NS0 mouse myeloma cell line transfected with mouse BMP‑6 and Recombinant Mouse BMP‑6 (Catalog # 6325-BM)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-6
References
- Chen, D. et al. (2004) Growth Factors 22:233.
- Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
- Inagaki, K. et al. (2006) Endocrinology 147:2681.
- Hong, J.H. et al. (2008) Immunology 128:e442.
- Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
- Saremba, S. et al. (2008) FEBS J. 275:172.
- Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
- Kersten, C. et al. (2005) BMC Immunol. 6:9.
- Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
- Shi, J. et al. (2009) Fertil. Steril. 92:1794.
- Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
- Zhang, Z. et al. (2006) Neuroscience 138:47.
- Dai, J. et al. (2005) Cancer Res. 65:8274.
- Meynard, D. et al. (2009) Nat. Genet. 41:478.
- Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.
Long Name
Bone Morphogenetic Protein 6
Alternate Names
BMP6, Vgr-1
Gene Symbol
BMP6
UniProt
Additional BMP-6 Products
Product Documents for Mouse BMP-6 Antibody
Product Specific Notices for Mouse BMP-6 Antibody
For research use only
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