Mouse Carbonic Anhydrase IX/CA9 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2344
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IX/CA9
Gln32-Asp390
Accession # Q8VHB5
Gln32-Asp390
Accession # Q8VHB5
Specificity
Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than1% cross‑reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Carbonic Anhydrase IX/CA9 Antibody
Carbonic Anhydrase IX in Mouse Colon.
Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Carbonic Anhydrase IX/CA9 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse colon
Sample: Perfusion fixed frozen sections of mouse colon
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA)
Sample: Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IX/CA9
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase IX (CA9), also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (2, 3). Recombinant mouse CA9 corresponds to the extracellular portion of mouse CA9.
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
- Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Alternate Names
CA9, G250, MN, RCC
Gene Symbol
CA9
UniProt
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Mouse Carbonic Anhydrase IX/CA9 Antibody
Product Specific Notices for Mouse Carbonic Anhydrase IX/CA9 Antibody
For research use only
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