Mouse Carbonic Anhydrase XII/CA12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2345
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 336103
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12
Ala25-Ser301
Accession # Q8CI85
Ala25-Ser301
Accession # Q8CI85
Specificity
Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, 100% cross‑reactivity with recombinant human (rh) CA12, 10% cross-reactivity with rhCA2, and no cross-reactivity with rhCA1, 3, 5A, 5B, 6, 7, 8, 10, 11, 13, recombinant mouse CA4, 9, or 14 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Carbonic Anhydrase XII/CA12 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)
Sample: Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase XII/CA12
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Mouse CA12 is a type I membrane enzyme with both CA activity and esterase activity (2, 3). The amino acid sequence of mouse CA12 shares 97%, 82%, and 68% identity with that of rat, human/chimpanzee/canine, and chicken.
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
- Okazaki, Y. et al. (2002) Nature 420:563.
Alternate Names
CA12
Gene Symbol
CA12
UniProt
Additional Carbonic Anhydrase XII/CA12 Products
Product Documents for Mouse Carbonic Anhydrase XII/CA12 Antibody
Product Specific Notices for Mouse Carbonic Anhydrase XII/CA12 Antibody
For research use only
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