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Mouse Cathepsin F Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2544

R&D Systems, part of Bio-Techne
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AF2544
AF2544-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin F
Ser20-Asn462
Accession # Q9R013

Specificity

Detects mouse Cathepsin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Cathepsin C, rmCathepsin H, and rmCathepsin L is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Cathepsin F Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse skeletal muscle and testis

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin F, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Cathepsin F
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin F

Cathepsin F is a lysosomal cysteine protease that participates in protein degradation and has been implicated in tumor progression.

Alternate Names

CTSF

Entrez Gene IDs

8722 (Human); 56464 (Mouse)

Gene Symbol

CTSF

UniProt

Additional Cathepsin F Products

Product Documents for Mouse Cathepsin F Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Cathepsin F Antibody

For research use only

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