Mouse Cathepsin H Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1013
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 175909
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin H
Glu22-Val333
Accession # Q3UCD6
Glu22-Val333
Accession # Q3UCD6
Specificity
Detects mouse Cathepsin H in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin H. In Western blots, no cross-reactivity with rmCathepsin A, D, E, X, and Z or recombinant human Cathepsin B, C, L, or S is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Mouse Cathepsin H Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Cathepsin H (Catalog # 1013-CY)
Sample: Recombinant Mouse Cathepsin H (Catalog # 1013-CY)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin H
Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).
References
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego.
- Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
- Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
- Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
- Guncar, G. et al. (1998) Structure 6:51.
- Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
- del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.
Alternate Names
CTSH
Gene Symbol
CTSH
UniProt
Additional Cathepsin H Products
Product Documents for Mouse Cathepsin H Antibody
Product Specific Notices for Mouse Cathepsin H Antibody
For research use only
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