Mouse CCL12/MCP-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB428
Key Product Details
Species Reactivity
Mouse
Applications
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 63205
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL12/MCP-5
Gly23-Gly104
Accession # Q62401.1
Gly23-Gly104
Accession # Q62401.1
Specificity
Detects mouse CCL12/MCP‑5 in ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL12/MCP-5 Antibody
Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody.
Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/ MCP-5 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB428). The ND50 is typically 0.5-2.5 µg/mL. Maximum neutralization observed was around 80%.Applications for Mouse CCL12/MCP-5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5)
Sample: Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5)
Neutralization
Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse CCL12/MCP‑5.
Mouse CCL12/MCP-5 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL12/MCP-5
CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.
References
- Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
- Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Alternate Names
MCP-5, MCP5
Entrez Gene IDs
20293 (Mouse)
Gene Symbol
CCL12
UniProt
Additional CCL12/MCP-5 Products
Product Documents for Mouse CCL12/MCP-5 Antibody
Product Specific Notices for Mouse CCL12/MCP-5 Antibody
For research use only
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