Mouse CCL12/MCP-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF428
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
Gly23-Gly104
Accession # Q62401
Gly23-Gly104
Accession # Q62401
Specificity
Detects mouse CCL12/MCP-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) MCP‑3 is observed and 5% cross-reactivity with recombinant mouse (rm) JE, rmMARC, rhMCP‑1, and rhMCP‑2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL12/MCP-5 Antibody
Chemotaxis Induced by CCL12/MCP-5 and Neutralization by Mouse CCL12/MCP-5 Antibody.
Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP-5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL12/MCP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428). The ND50 is typically 0.8-4.0 µg/mL.Applications for Mouse CCL12/MCP-5 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5)
Sample: Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5)
Neutralization
Measured by its ability to neutralize CCL12/MCP-5-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP-5.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF428 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL12/MCP-5
CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.
References
- Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
- Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Alternate Names
MCP-5, MCP5
Entrez Gene IDs
20293 (Mouse)
Gene Symbol
CCL12
UniProt
Additional CCL12/MCP-5 Products
Product Documents for Mouse CCL12/MCP-5 Antibody
Product Specific Notices for Mouse CCL12/MCP-5 Antibody
For research use only
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