Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

References

Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
Gu, L. et al. (1999) Chem. Immunol. 72:7.
Luini, W. et al. (1994) Cytokine 6:28.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

P10148

Additional CCL2/JE/MCP-1 Products

Product Documents for Mouse CCL2/JE/MCP-1 Alexa Fluor® 405-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCL2/JE/MCP-1 Alexa Fluor® 405-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CCL2/JE/MCP-1 Alexa Fluor® 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4791V

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CCL2/JE/MCP-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Mouse CCL2/JE/MCP-1 Alexa Fluor® 405-conjugated Antibody

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: CCL2/JE/MCP-1