Mouse CCL2/JE/MCP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB479
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Neutralization
Cited:
Bioassay, Immunohistochemistry, Immunohistochemistry-Frozen, In vivo assay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 123616
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # P10148
Gln24-Arg96
Accession # P10148
Specificity
Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human
(rh) CCL1, 2, 3,
4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,
rmCCL1, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12,
17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL2/JE/MCP-1 Antibody
Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody.
Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.Applications for Mouse CCL2/JE/MCP-1 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL2/JE/MCP-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP-1.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
References
- Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
- Gu, L. et al. (1999) Chem. Immunol. 72:7.
- Luini, W. et al. (1994) Cytokine 6:28.
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
UniProt
Additional CCL2/JE/MCP-1 Products
Product Documents for Mouse CCL2/JE/MCP-1 Antibody
Product Specific Notices for Mouse CCL2/JE/MCP-1 Antibody
For research use only
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