Mouse CCL2/JE/MCP‑1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF479
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Detection, ELISA Development, ELISA Development (Detection), Immunohistochemistry, Immunohistochemistry-Paraffin, Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1 (R&D Systems, Catalog # 479-JE)
Gln24-Arg96
Accession # Q5SVU3
Gln24-Arg96
Accession # Q5SVU3
Specificity
Detects mouse CCL2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross‑reactivity with rhMCP‑1, rhMCP‑2, rhMCP‑3, rmMARC, rhMCP‑4, rmMCP‑5, rmMDC, rmMIP‑1a, rmMIP‑1b, rmMIP‑1g, rmMIP‑2, rmMIP‑3b, rmRANTES, rmTARC and rmTECK is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE)
Sample: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE)
Mouse CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using BAF479 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
References
- Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
- Gu, L. et al. (1999) Chem. Immunol. 72:7.
- Luini, W. et al. (1994) Cytokine 6:28.
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
UniProt
Additional CCL2/JE/MCP-1 Products
Product Documents for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody
Product Specific Notices for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody
For research use only
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