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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Cited:

ELISA Development, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 59106

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL21/6Ckine
Ser24-Gly133
Accession # P84444

Specificity

Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 6Ckine, rhMIP-1 alpha, rhMIP-1 beta, recombinant mouse (rm) MIP-1 alpha, rmMIP-1 beta, rhMIP-1 delta, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rhTeck, or rmTeck.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCL21/6Ckine Antibody

CCL21/6Ckine antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).

CCL21/6Ckine in Mouse Thymus.

CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of CCL21/6Ckine antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse CCL21/6Ckine Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

25 µg/mL
Sample: Perfusion fixed frozen sections of normal mouse thymus, intestine, liver, and kidney

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample:

D3 mouse embryonic stem cell line fixed with paraformaldehyde and permeabilized with saponin.

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C)

Mouse CCL21/6Ckine Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL21/6Ckine Biotinylated Antibody (Catalog # BAF457)
  • Standard: Recombinant Mouse CCL21/6Ckine Protein (Catalog # 457-6C)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB457 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL21/6Ckine

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid, highly basic, precursor protein with a 23 aa signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid protein with a 23 signal peptide that is cleaved to generate the 110 aa mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% aa sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta/ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines.

References

  1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
  2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
  3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.

Alternate Names

6Ckine, exodus-2, SCYA21, SLC, TCA-4

Entrez Gene IDs

6366 (Human); 18829 (Mouse); 298006 (Rat)

Gene Symbol

CCL21

UniProt

Additional CCL21/6Ckine Products

Product Documents for Mouse CCL21/6Ckine Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCL21/6Ckine Antibody

For research use only

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