Mouse CCL28 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB533
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunohistochemistry-Frozen, Immunoprecipitation, In vivo assay, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 134306
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Ile23-Arg130
Accession # Q9JIL2
Specificity
Detects mouse CCL28 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) CCL28 is observed and no cross-reactivity with rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16,
17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7,
9/10, 11, 12, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL28 Antibody
Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody.
Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL28 Monoclonal Antibody (Catalog # MAB533). The ND50 is typically 2.5-5.0 µg/mL.Applications for Mouse CCL28 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL28 (Catalog # 533-VI) under non-reducing conditions only
Sample: Recombinant Mouse CCL28 (Catalog # 533-VI) under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR10. The Neutralization Dose (ND50) is typically 2.5-5.0 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL28.
Reviewed Applications
Read 1 review rated 4 using MAB533 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL28
Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.
References
- Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Alternate Names
CCL28, MEC, VIC
Gene Symbol
CCL28
UniProt
Additional CCL28 Products
Product Documents for Mouse CCL28 Antibody
Product Specific Notices for Mouse CCL28 Antibody
For research use only
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