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Mouse CCL28 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5331

R&D Systems, part of Bio-Techne
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MAB5331
MAB5331-SP

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 134315

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2

Specificity

Detects mouse CCL28 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human CCL28 or recombinant mouse CCL27 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CCL28 Antibody

Mouse CCL28 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL28 Biotinylated Antibody (Catalog # BAF533)
  • Standard: Recombinant Mouse CCL28 Protein (Catalog # 533-VI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) precursor protein with a 22 aa signal peptide and a 108 aa mature protein. Mature human and mouse CCL28 share 83% aa identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10, which is also the receptor for CCL27/CTACK.

References

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Alternate Names

CCL28, MEC, VIC

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Gene Symbol

CCL28

UniProt

Additional CCL28 Products

Product Documents for Mouse CCL28 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCL28 Antibody

For research use only

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