Mouse CCL5/RANTES Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB478
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Neutralization
Cited:
Bioassay, ELISA Development, ELISA Development (Capture), Immunocytochemistry, Immunohistochemistry-Frozen, In vivo assay, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 53405
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL5/RANTES
Ser24-Ser91
Accession # P30882
Ser24-Ser91
Accession # P30882
Specificity
Detects mouse CCL5/RANTES in direct ELISAs. Shows 50% cross-reactivity with recombinant human (rh) CCL5, and no cross-reactivity with recombinant feline CCL5, rhCCL3, recombinant mouse (rm) CCL3, rmCCL4, rhCCL4, rhCCL14, or rhCCL18.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL5/RANTES Antibody
CCL5/RANTES in Mouse Splenocytes.
CCL5/RANTES was detected in immersion fixed mouse splenocytes using Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody.
Recombinant Mouse CCL5/RANTES (Catalog # 478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478). The ND50 is typically 0.1-0.5 µg/mL.Detection of Mouse CCL5/RANTES by Immunohistochemistry
Antagonizing RANTES within CNS extends the survival of mice after TBEV infection. Mice were treated with Met-RANTES, vehicle, anti-RANTES mAb, or isotype-matched control Ab daily from day 2 to 8 after a primary TBEV infection. a Met-RANTES-treated mice exhibited a delay in mortality following lethal TBEV challenge. Mortality in each group was monitored daily for 14 days. Data were pooled from three independent experiments (total of approximately 18 mice per group). Statistical differences were evaluated using the Kaplan–Meier test for mortality. *P < 0.05, compared to vehicle-treated mice. b Anti-RANTES mAb-treated mice exhibited a delay in mortality following lethal TBEV challenge. Mortality in each group was monitored daily for 14 days. Data were pooled from three independent experiments (total of approximately 18 mice per group). *P < 0.05, compared to isotype Ab-treated mice. c, d Body weight changes of mice were monitored daily. For each time point, the measured values are the average of the surviving mice. Bars represent the means ± the standard deviations of three independent experiments (total of approximately 18 mice per group). e Infectious virus in brain tissues. No significant difference in virus titers was observed at day 2, 5, or 8 between Met-RANTES-treated mice and vehicle-injected mice or anti-RANTES mAb-treated and isotype Ab-injected mice. Bars represent the means ± the standard deviations of three independent experiments (n = 4 mice per group). NS not significant. f Met-RANTES or anti-RANTES mAb treatment reduced inflammatory cell accumulation (indicated by black arrows) in cerebral cortex sections, compared to results for vehicle- or isotype Ab-treated mice, respectively. The histopathological changes of PFA-fixed sections in cerebral cortex were examined by HE staining on day 8 p.i. The photomicrographs demonstrate representative images obtained from three independent experiments (n = 4 mice per group). Bars, 100 μm Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0665-9), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CCL5/RANTES Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse polymorphonuclear cells and mouse splenocytes
Sample: Immersion fixed mouse polymorphonuclear cells and mouse splenocytes
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.025 µg/mL Recombinant Mouse CCL5/RANTES.
Reviewed Applications
Read 3 reviews rated 4.7 using MAB478 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
References
- Schall, T.J. et al. (1990) Nature 347:669.
- Bacon, K.B. et al. (1995) Science 269:1727.
- Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
- Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
- Heeger, P. et al. (1992) Kidney Int. 41:220.
- Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
- Levy, J.A. (2009) J. Immunol. 182:3945.
- Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
- DeVico, A.L. and R.C. Gallo (2004) Nat. Rev. Microbiol. 2:401.
- Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
- Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
- Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885.
- von Hundelshausen, P. et al. (2005) Blood 105:924.
- von Hundelshausen, P. et al. (2001) Circulation 103:1772.
- Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
- Baltus, T. et al. (2003) Blood 102:1985.
- Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Alternate Names
RANTES, SISd
Gene Symbol
CCL5
UniProt
Additional CCL5/RANTES Products
Product Documents for Mouse CCL5/RANTES Antibody
Product Specific Notices for Mouse CCL5/RANTES Antibody
For research use only
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