Mouse CCR3 Fluorescein-conjugated Antibody

Detection of CCR3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB729F) and Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A). Quadrant markers were set based on control antibody staining (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Detection of Mouse CCR3 by Flow Cytometry

Gal-3 deficient Eos exhibit decreased migration toward eotaxin-1. (A) Migration of WT and Gal-3-/- BM-derived Eos toward murine eotaxin-1 (100 nM) in vitro using 96-well Transwell ® Chambers. Results are represented as percent cell migration relative to WT Eos. (B) Expression of CCR3 by WT and Gal-3-/- Eos by flow cytometry using FITC-conjugated rat anti-mouse CCR3 with FITC-conjugated rat IgG2a as isotype control. Representative data of two independent experiments with Eos from different mice is shown. (C) Migration of WT Eos suspended in medium alone or medium containing lactose or maltose toward murine eotaxin-1. Number of cells that migrated in each case was determined and expressed as the average number of cells/field. Combined data (mean ± SEM) from three independent experiments in duplicate is shown in (A) and (C). #p <0.05 versus WT Eos in (A). Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fphar.2013.00037/abstract), licensed under a CC-BY license. Not internally tested by R&D Systems.