Mouse CCR5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6138
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCR5
Met1-Ala32, Ala92-Lys104, Thr169-Met200, Gln263-Gln279 (Ile11Ser, Ile97Val)
Accession # P51682
Met1-Ala32, Ala92-Lys104, Thr169-Met200, Gln263-Gln279 (Ile11Ser, Ile97Val)
Accession # P51682
Specificity
Detects mouse CCR5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CCR5 Antibody
Detection of Mouse CCR5 by Western Blot.
Western blot shows lysates of EL-4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CCR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6138) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse CCR5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: EL‑4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue
Sample: EL‑4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR5
CCR5 (CC chemokine receptor 5; also CD195 and MIP-1 alpha receptor) is a 41kDa (predicted) member of the G-protein coupled receptor #1 family of proteins. It is expressed on Treg T cells, NK cells, neurons, macrophages and Th1 T cells. CCR5 responds to RANTES, MIP-1 alpha and -1 beta, and induces cell adhesion and migration. Mouse CCR5 is a 7-transmembrane glycoprotein 354 amino acids (aa) in length and it contains an O-glycosylated and sulfated extracellular N-terminus (aa 1-32), plus a phosphorylated and palmitoylated intracellular C-terminus (aa 304-354). In human and primates, CCR5 appears as a 62-64 kDa membrane form, possibly due to extensive posttranslational modification. CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Over aa 1-32, 92-104, 169-200 and 263-279 collectively, mouse CCR5 shares 88% and 78% aa identity with rat and human CCR5, respectively.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
UniProt
Additional CCR5 Products
Product Documents for Mouse CCR5 Antibody
Product Specific Notices for Mouse CCR5 Antibody
For research use only
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