HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP was stained with either (A) Rabbit Anti-Mouse CCR8 Monoclonal Antibody (Catalog # MAB8324) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Applications for Mouse CCR8 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using MAB8324 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR8

CCR8 (C-C chemokine Receptor 8; also known as CD198) is a 41-43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8⁺ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O-glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. Over aa sequences 1-33 and 92-105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P56484

Additional CCR8 Products

Product Documents for Mouse CCR8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCR8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CCR8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8324

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Mouse CCR8 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CCR8 Antibody

Detection of CCR8 in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP by Flow Cytometry.