RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCRL2/L-CCR Monoclonal Antibody (Catalog # MAB5519, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

CCRL2/L‑CCR/CRAM‑A/B in RAW 264.7 Mouse Cell Line.

CCRL2/L-CCR/CRAM-A/B was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse CCRL2/L-CCR/CRAM-A/B Monoclonal Antibody (Catalog # MAB5519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CCRL2/LCCR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCRL2/LCCR

Lipopolysaccharide-inducible CC chemokine receptor (LCCR), also known as CCRL2, is a 7TM chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49% and 70% aa sequence identity with human and rat LCCR, respectively.

Long Name

CC Chemokine Receptor-like 2

Alternate Names

LCCR

Entrez Gene IDs

54199 (Mouse)

Gene Symbol

CCRL2

UniProt

O35457

Additional CCRL2/LCCR Products

Product Documents for Mouse CCRL2/LCCR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCRL2/LCCR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CCRL2/LCCR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5519

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CCRL2/LCCR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CCRL2/LCCR Antibody

Detection of CCRL2/L‑CCR in RAW 264.7 Mouse Cell Line by Flow Cytometry.