Mouse CD117/c-kit Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1356

R&D Systems, part of Bio-Techne
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BAF1356
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AF1356
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532

Specificity

Detects mouse CD117/c-kit in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human CD117 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CD117/c-kit Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Lineage depleted mouse bone marrow

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD117/c-kit
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P05532

Additional CD117/c-kit Products

Product Documents for Mouse CD117/c-kit Biotinylated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD117/c-kit Biotinylated Antibody

For research use only

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