Mouse C57BL/6 splenocytes were stained with (A) Mouse Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB76141A) or (B) Mouse IgG2A Isotype Control (IC003A) and Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Mouse CD161/NK1.1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: C57BL/6 splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Entrez Gene IDs

17059 (Mouse)

Gene Symbol

KLRB1C

UniProt

AAH61168

Additional CD161/NK1.1 Products

Product Documents for Mouse CD161/NK1.1 APC-conjugated Antibody

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Product Specific Notices for Mouse CD161/NK1.1 APC-conjugated Antibody

For research use only

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Flow Cytometry

Mouse CD161/NK1.1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB76141A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD161/NK1.1 APC-conjugated Antibody

Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry.