Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rabbit Anti-Mouse CD177 PE-conjugated Monoclonal Antibody (Catalog # FAB8186P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC105P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD177 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse bone marrow cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD177

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References

Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
Klippel, S. et al. (2002) Blood 100:2441.
Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
Temerinac, S. et al. (2000) Blood 95:2569.
Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
von Vietinghoff, S. et al. (2007) Blood 109:4487.
Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human); 68891 (Mouse)

Gene Symbol

CD177

UniProt

Q8R2S8

Additional CD177 Products

Product Documents for Mouse CD177 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse CD177 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CD177 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8186P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD177 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD177 PE-conjugated Antibody

Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry.