Mouse CD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2828
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD2
Arg23-Ser203
Accession # P08920
Arg23-Ser203
Accession # P08920
Specificity
Detects mouse CD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human CD2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CD2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse CD3+ splenocytes
Sample: Mouse CD3+ splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD2
Sample: Recombinant Mouse CD2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD2
Mouse CD2, also known as LFA-2 and LFA-3 Receptor, is a 55‑66 kDa, variably glycosylated type I transmembrane (TM) protein belonging to the CD2 receptor subset of the Ig superfamily. It is synthesized as a 344 amino acid (aa) precursor that contains a 22 aa signal sequence, a 181 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a proline rich (20%) 115 aa cytoplasmic region. CD2 is found on T and NK cells and binds CD48 in rodents and CD58 in humans. The ECD of mouse CD2 shares 73%, 50% and 44% aa identity with the CD2 ECD in rat, human and equine, respectively.
Alternate Names
CD2, LFA-2, T11
Gene Symbol
CD2
UniProt
Additional CD2 Products
Product Documents for Mouse CD2 Antibody
Product Specific Notices for Mouse CD2 Antibody
For research use only
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