Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, recombinant mouse LMIR3, 4, or 5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse CD300a/LMIR1 PE-conjugated Antibody

Detection of CD300a/LMIR1 in T1165 Mouse Cell Line by Flow Cytometry.

T1165 mouse plasmacytoma cell line was stained with Rat Anti-Mouse CD300a/LMIR1 PE-conjugated Monoclonal Antibody (Catalog # FAB1186P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD300a/LMIR1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: T1165 mouse plasmacytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD300a/LMIR1

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References

Clark, G.J. et al. (2009) Trends Immunol. 30:209.
Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
Munitz, A. et al. (2006) Blood 107:1996.
Bachelet, I. et al. (2005) J. Immunol. 175:7989.
Bachelet, I. et al. (2008) J. Immunol. 180:6064.
Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Gene Symbol

CD300A

UniProt

BAC80268

Additional CD300a/LMIR1 Products

Product Documents for Mouse CD300a/LMIR1 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CD300a/LMIR1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CD300a/LMIR1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1186P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications