Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1866
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Extracellular Domain
Gln19-Pro158
Accession # NP_034711
Gln19-Pro158
Accession # NP_034711
Specificity
Detects mouse CD47 N-terminal IgV-like Extracellular Domain in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human CD47 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary and thymus
Sample: Perfusion fixed frozen sections of mouse ovary and thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD47 N-terminal IgV-like Extracellular Domain
Sample: Recombinant Mouse CD47 N-terminal IgV-like Extracellular Domain
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD47
CD47, also known as integrin-associated protein, is an integral membrane protein with a single IgV-like domain at its N-terminus followed by five membrane-spanning regions. CD47 is expressed ubiquitously. It is a ligand for the transmembrane signal regulatory protein alpha 1 (SIRP alpha) and also functions as a receptor for thrombospondin.
Alternate Names
CD47, IAP, MER6, OA3
Entrez Gene IDs
Gene Symbol
CD47
UniProt
Additional CD47 Products
Product Specific Notices
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...