Mouse CD55/DAF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5376
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF
Asp35-Pro359
Accession # Q61475
Asp35-Pro359
Accession # Q61475
Specificity
Detects mouse CD55/DAF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CD55 and recombinant mouse CD97 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CD55/DAF Antibody
Detection of Mouse CD55/DAF by Western Blot.
Western blot shows lysates of mouse spleen tissue and mouse splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Detection of Mouse CD55/DAF by Western Blot
Glimepiride releases CD14 from RAW 264 cells. (A) The amounts of CD14 in RAW 264 cells treated for one hour with control medium (□) or glimepiride (■) as shown. Values are means ± SD, from triplicate experiments performed 4 times, n = 12. (B) The amounts of CD14 in supernatants from RAW 264 cells treated for one hour with control medium (□) or glimepiride as shown (■). Values are means ± SD, from triplicate experiments performed 4 times, n = 12. (C) Immunoblots showing the amounts of CD14, PrPC, CD55 and caveolin in extracts from RAW 264 cells treated for 1 hour with control medium (i) or 5 μM glimepiride (ii). (D) The amounts of CD14 in cells (□) or supernatants (■) from microglial cells treated for 1 hour with control medium, 5 μM glimepiride or 5 μM glipizide. Values are mean units CD14 ± SD, from triplicate experiments performed 3 times, n = 9. *Cellular CD14 significantly less than control cells. **supernatant CD14 significantly greater than control supernatants. (E) Blot showing the amounts of CD14 in supernatants from microglial cells treated with concentrations of glimepiride as shown for one hour. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24952384), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CD55/DAF Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Western Blot
1 µg/mL
Sample: Mouse spleen tissue and Mouse splenocytes
Sample: Mouse spleen tissue and Mouse splenocytes
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD55/DAF
CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules. It is found on blood cells, epithelium and endothelium and serves both as a receptor for CD97 and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-60 kDa, 356 amino acid (aa), GPI-linked form that is ubiquitously expressed. This molecule contains four SUSHI domains (aa 35-285), a Ser/Thr-rich region (aa 288-362) and a GPI-anchor at Gly362. There is also a 50 kDa, 379 aa, type I transmembrane form that is testis-associated. It shows the same domain architecture and is 93% aa identical to the GPI-form. At least four GPI gene isoforms exist. They diverge after Ile285 and show deletions and substitutions. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.
Alternate Names
CD55, CR, DAF, TC
Gene Symbol
CD55
UniProt
Additional CD55/DAF Products
Product Documents for Mouse CD55/DAF Antibody
Product Specific Notices for Mouse CD55/DAF Antibody
For research use only
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