Mouse CD69 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2386

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Affinity Purification

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 310106

View all CD69 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217

Specificity

Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse CD69 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: CTLL-2 mouse cytotoxic T cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD69 under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

P37217

Additional CD69 Products

Product Documents for Mouse CD69 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD69 Antibody

For research use only

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