Mouse CDNF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5187

R&D Systems, part of Bio-Techne
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MAB5187
MAB5187-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 560108

View all CDNF Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CDNF
Leu27-Leu187
Accession # Q8CC36

Specificity

Detects mouse CDNF in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CDNF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CDNF Antibody

CDNF antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

CDNF in Mouse Brain.

CDNF was detected in perfusion fixed frozen sections of mouse brain using Rat Anti-Mouse CDNF Monoclonal Antibody (Catalog # MAB5187) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse CDNF Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDNF

CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1), is a 17‑19 kDa secreted protein that shares 52% amino acid (aa) identity with mouse MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet (1). The Armet designation is not preferred, because the proteins as translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfide bonds, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1‑3). The mouse CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature mouse CDNF shares 80%, 87%, 83% and 82% aa identity with human, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre and postnatal mouse brain, they are mostly abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson's disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).

References

  1. Lindholm, P. et al. (2007) Nature 448:73.
  2. Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
  3. Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
  4. Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.

Long Name

Conserved Dopamine Neurotrophic Factor

Alternate Names

ARMETL1

Entrez Gene IDs

441549 (Human); 227526 (Mouse); 361276 (Rat)

Gene Symbol

CDNF

UniProt

Q8CC36 (Mouse)

Additional CDNF Products

Product Documents for Mouse CDNF Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CDNF Antibody

For research use only

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